Every morning and every night. I also carry a toothbrush with me because when I'm out all day, I often have to stop and eat. I don't want to be one of those people who walk around with foul breath and chunks of their last lunch in thêir teeth. I always found flossing difficult til they came out with those flossing tapes, though, because my teeth are not spaced widely enough. Super uncomfortable, and the floss would get stuck. Love flossing tape, though. That stuff really works! I have never used mouthwash and never will. Especially ones with triclosan in them. There's quite a bit of debate of what mouthwash does to the natural, necessary bacteria in your mouth, and have seen articles on the possibility of mouthwashes causing oral cancers. If I'm feeling like my breath isn't fresh, I brush, immediately. Nothing worse than having toilet-bowl-mouth. Especially if a person smokes and drinks coffee/tea. Which I do, unfortunately. Not only does it taste disgusting all day if I don't brush, I can just imagine the skanky smell ** gaaag** No excuse for being dirty or smelly when a person doesn't have to be.